백서의 두개골 결손부에서 제대혈 유래 간엽줄기세포의 골재생에 관한 연구
Bone Regeneration of Mesenchymal Stem Cells Isolated from Umbilical Cord Blood in Calvarial Defect of Athymic Mouse
윤정현, 이진용, 임재형, 이의석, 서병무, 장현석, 권종진, 임재석,
소속 상세정보
윤정현 ( Yoon Jung-Hyun ) - 고려대학교 구강악안면외과학교실
이진용 ( Lee Jin-Yong ) - 고려대학교 구강악안면외과학교실
임재형 ( Lim Jae-Hyung ) - 고려대학교 구강악안면외과학교실
이의석 ( Lee Eui-Seok ) - 고려대학교 구강악안면외과학교실
서병무 ( Seo Byoung-Moo ) - 서울대학교 구강악안면외과학교실
장현석 ( Jang Hyun-Seok ) - 고려대학교 구강악안면외과학교실
권종진 ( Kwon Jong-Jin ) - 고려대학교 구강악안면외과학교실
임재석 ( Rim Jae-Suk ) - 고려대학교 구강악안면외과학교실
KMID : 1146620130020010001
Abstract
It has been suggested that umbilical cord blood (UCB) contains multipotent stem cells. Some authors suggest that within UCB are cells with potential for differentiation down mesenchymal lineages and UCB is one of the readily available sources of stem cells that may be used for bone regeneration. Isolation and characterization of these cells has been accomplished in some centers. Differentiation of these cells down multiple lineages has been documented. The use of UCB derived stem cells was investigated in this animal studies. Polycaprolactone?20% tricalcium phosphate (PCL?TCP) polymers allow for cellular adherence and differentiation and are adequate scaffolds for bone regeneration in tissue engineering strategies. The purpose of this study was to evaluate the potential of MSCs from UCB seeded onto PCL?TCP composite scaffolds for bone tissue engineering. MSCs were isolated from UCB. MSCs from UCB seeded onto PCL?TCP scaffolds were then implanted in calvarial bone defect of the athymic mouse. As a control, in each mouse was treated with scaffold only. At six and twelve weeks post?implantation, histological examinations were performed. MSCs from UCB with PCL?TCP scaffolds did not exhibited bone regeneration in calvarial bone defects in athymic mouse. Histological examination did not reveal the presence of regenerated bone of the defect. MSCs from UCB should be considered further applications in tissue?engineered bone regeneration.
키워드
Umbilical cord blood derived mesenchymal stem cells; Mesenchymal stem cells; Umbilical cord blood; PCL?TCP scaffold; Bone regeneration; Athymic mouse
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